How this calculator works
Enter molar absorptivity (l/mol·cm), path length (cm), concentration (mol/l). CalcStack applies the displayed formula and reports each intermediate planning value with the units shown.
Formula / method
A = εlc
Worked example
ε 15,000, 1 cm path length and 20 μM concentration yield absorbance 0.3.
Assumptions and limitations
The sample is homogeneous, monochromatic light is used and Beer-Lambert linearity holds.
Scattering, high concentration, stray light and chemical changes can break linearity.